her2 breast cancer cell lines uacc812 (ATCC)
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Her2 Breast Cancer Cell Lines Uacc812, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 166 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 166 article reviews
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1) Product Images from "Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer"
Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer
Journal: Disease Markers
doi: 10.1155/2020/6103542
Figure Legend Snippet: Associations between SGLT1 protein expression levels and clinicopathologic variables of the breast cancer patient cohort.
Techniques Used: Expressing
Figure Legend Snippet: Representative staining of SGLT1 in different subtypes of breast cancer (×100, ×400). (a) Negative immunoreactions. Scale bar: 100 μ m. (b) Positive staining of SGLT1 in luminal-like breast cancer. (c) Positive staining of SGLT1 in HER2+ breast cancer. (d) Positive staining of SGLT1 in basal-like breast cancer.
Techniques Used: Staining
Figure Legend Snippet: Representative western blot of HER2 and SGLT1 expression levels and quantification relative to actin in UACC812 and SKBR3 cells. UACC812 and SKBR3 cells were treated with HER2 inhibitors (HER2 I), Phlorizin, MG132 alone, or in combination for 48 h. Untreated cells were used as a negative control (NC). (a) A representative WB detection result of three independent experiments was shown. (b) The relative levels of HER2 and SGLT1 protein in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05.
Techniques Used: Western Blot, Expressing, Negative Control
Figure Legend Snippet: The curve and column results of the ECAR values for UACC812 and SKBR3 cells. (a) The ECAR values of UACC812 and SKBR3 cells treated with 0, 10, 20, 30, 40, 50, and 60 min, respectively. (b) The ECAR values of each group at 40 min in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01.
Techniques Used:
Figure Legend Snippet: Representative western blot of PI3K/Akt/mTOR pathway-related protein expression levels and quantification relative to actin in UACC812 cells. A representative WB detection result of PI3K/Akt/mTOR pathway-related protein expression levels in (a) SGLT1 siRNA and (d) phlorizin treatment. The relative levels of PI3K/Akt/mTOR protein in (b) SGLT1 siRNA and (e) phlorizin treatment. The relative levels of p-PI3K/p-Akt/p-mTOR protein in (c) SGLT1 siRNA and (f) phlorizin treatment. The results shown were representative of at least three independent experiments; ∗∗∗∗ p < 0.0001.
Techniques Used: Western Blot, Expressing
Figure Legend Snippet: CCK-8 assays for the effect of SGLT1 expression on cell proliferation in UACC812 and SKBR3 cells. (a) The cell viability of UACC812 and SKBR3 cells relative to negative control groups after 0, 12, 24, 36, and 48 h treatment, respectively. (b) The cell viability of each group relative to negative control groups at 48 h in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05.
Techniques Used: CCK-8 Assay, Expressing, Negative Control
Figure Legend Snippet: Cox's univariate regression analysis of the patient survival in breast cancer with respect to clinical parameters and cancer biological factors.
Techniques Used:
Figure Legend Snippet: Kaplan-Meier survival analysis according to SGLT1 expression in the whole cohort and HER2+ subtype of breast cancer patients (log-rank test). Association of SGLT1 expression levels with (a) DFS and (c) OS in the whole cohort of breast cancer patients. Association of SGLT1 expression levels with (b) DFS and (d) OS in HER2+ breast cancer patients.
Techniques Used: Expressing
Figure Legend Snippet: Kaplan-Meier survival analysis according to SGLT1 expression in basal-like, luminal-like, and luminal-HER2+ subtype of breast cancer patients (log-rank test). Association of SGLT1 expression levels with (a) DFS and (d) OS in basal-like breast cancer patients. Association of SGLT1 expression levels with (b) DFS and (e) OS in luminal-like breast cancer patients. Association of SGLT1 expression levels with (c) DFS and (f) OS in LuminalHER2+ breast cancer patients.
Techniques Used: Expressing
Figure Legend Snippet: Cox's multivariate regression analysis of the patient survival in breast cancer with respect to clinical parameters and cancer biological factors.
Techniques Used:
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